Fig. 3. Effects of epinephrine in T cells of mice with CIA on Th17 and Treg cell differentiation and function. The activated T cells were incubated with epinephrine for 24 h and labeled simultaneously with anti-CD4, anti-CD25 and anti-IL-17 or anti-Foxp3 fluorescence antibodies. The CD4⁺ cells were firstly selected, and then percentage of Th17 (CD25⁻IL-17⁺) and Treg (CD25⁺Foxp3⁺) cells in CD4⁺ T cells was analyzed (A). Levels of ROR-γt and Foxp3 protein expression (B) and IL-17, IL-22, TGF-β and IL-10 mRNA expression (C) in T cells as well as IL-17, IL-22, TGF-β1 and IL-10 level in the supernatants of T cell cultures (D) were assessed. E=epinephrine; ^**p<0.01, versus intact control; ⁺⁺p<0.01, versus CIA group; ^#p<0.05, ^##p<0.01, versus CIA + E (10^-6 M) group.